Evaluation of different rehydration methods in the course of preparation of tendon samples.

Biomechanical tests aim the understanding of the structure-function relationship often require time-consuming preparation of the samples. During preparation of the tendon samples, occurs an unfavorable effect of sample drying that leads to ultimate dehydration. To invert the impact of tissue samples dehydration, one can use different buffer solutions. Although, buffer solutions may alter the physiology of the tissue, disturbing the result of the mechanical tests. The nature of these changes depends on the osmotic pressure of applied solution. The osmotic effects are well described in the literature but processes of tissue dehydration are still poorly understood. This study aimed to evaluate the influence of hyper, hypo and isotonic solutions on the rehydration process and resulting biomechanical properties of tendon samples.